DNA

Part:BBa_K4409007:Design

Designed by: Kexin Fei   Group: iGEM22_Worldshaper-HZ   (2022-09-20)


dsDNA(hsa_circ_0001785)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This dsDNA needs to be used with the guide sequence BBa_K4409001 in a gRNA. The dsDNA also contains a PAM site which is required for a Cas nuclease to cut. In the Cas12 protein, the sequence for the PAM site is TTTX, so we used TTTA.


Source

This part is based on the hsa_circ_0001785 sequence retrieved from circBank database.

References