DNA
Part:BBa_K4409007:Design
Designed by: Kexin Fei Group: iGEM22_Worldshaper-HZ (2022-09-20)
dsDNA(hsa_circ_0001785)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This dsDNA needs to be used with the guide sequence BBa_K4409001 in a gRNA. The dsDNA also contains a PAM site which is required for a Cas nuclease to cut. In the Cas12 protein, the sequence for the PAM site is TTTX, so we used TTTA.
Source
This part is based on the hsa_circ_0001785 sequence retrieved from circBank database.